Abstract
CLONING of DNA fragments is a new powerful technique in molecular genetics and a number of interesting experiments involving both prokaryotic and eukaryotic genomes have already been reported (see ref. 1 for review). We present here results concerning the cloning of all EcoRI (ref. 2) fragments from phage λ DNA in Escherichia coli using as a vector plasmid pSC101 (ref. 3). To achieve this, we had to solve the problem of propagating the λ fragment containing the immunity region since the phage strain used had a temperature-sensitive mutation in that region.
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BERNARDI, A., BERNARDI, G. Cloning of all EcoRI fragments from phage λ in E. coli. Nature 264, 89–90 (1976). https://doi.org/10.1038/264089a0
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DOI: https://doi.org/10.1038/264089a0
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