Abstract
WHEN the mixture of products obtained by combining insulin A and B chains in solution (for a review, see ref. 1) was chromatographed on an anti-insulin antibody–Sepharose column2 no clear separation was achieved3. From the low biological activity (24.1%) of the material with the same antibody affinity as natural insulin (10.9% of the total bound products) it was concluded3 that resynthesised insulin with authentic structure constituted only a small portion of this fraction. Combination of insulin chains in solution has always involved a number of steps4,5 and has produced a mixture of products from which pure active insulin could be isolated by gel filtration and ion–exchange chromatography6. Linking the chains using the procedure described in this report, however, has given only one combination product that, according to various criteria, was identical with insulin and could be separated from unreacted A and B chains in one step.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Lübke, K., and Klostermeyer, H., Adv. Enzym. rel. Areas molec. Biol., 33, 445–525 (1970).
Akanuma, Y., Kuzuya, T., Hayashi, M., Ide, T., and Kuzuya, N., Biochem. biophys. Res. Commun., 38, 947–953 (1970).
Klotz, M., Diplomarbeit, Univ. Köln (1974).
Brandenburg, D., Schermutzki, W., and Zahn, H., Hoppe-Seyler's Z. physiol. Chem., 354, 1521–1524 (1973).
Geiger, R., and Obermeier, R., Biochem. biophys. Res. Commun., 55, 60–66 (1973).
Rüegg, U. T., and Gattner, H.-G., Hoppe-Seyler's Z. physiol. Chem., 356, 1527–1533 (1975).
Yagi, Y., Maier, P., and Pressman, D., Science, 147, 617–619 (1965).
Gutte, B., thesis, Technische Hochschule Aachen (1967).
Ouchterlony, O., Prog. Allergy, 5, 1–78 (1958).
Levy, H. B., and Sober, H. A., Proc. Soc. exp. Biol. Med., 103, 250–252 (1960).
Axen, R., Porath, J., and Ernback, S., Nature, 214, 1302–1304 (1967).
Ellman, G. L., Archs Biochem. Biophys., 82, 70–77 (1959).
Fruchter, R. G., and Crestfield, A. M., J. biol. Chem., 240, 3868–3874 (1965).
Du, Y.-c., Zhang, Y.-s., Lu, Z.-x., and Tsou, C.-l., Scientia Sinica, 10, 84–104 (1961).
Brown, H., Sanger, F., and Kitai, R., Biochem. J., 60, 556–565 (1955).
Rodbell, M., J. biol. Chem., 239, 375–380 (1964).
Gliemann, J., Diabetologia, 3, 382–388 (1967).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KLOTZ, M., GUTTE, B. Combination of insulin chains on an anti-insulin antibody–Sepharose column. Nature 262, 791–793 (1976). https://doi.org/10.1038/262791a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/262791a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.