Abstract
ASSEMBLY of cell-surface components at one pole of the cell membrane, as a result of cross linkage with specific ligands, can be visualised by using labelled ligands. Such a demonstration of topographic changes on the cell surface is called capping. By using different labels for the different ligands it is possible to study the mutual relationship between various membrane components. Using the capping technique, it was possible to demonstrate the relationship between concanavalin A (con A) receptors and immunoglobulin (Ig) receptors on the lymphocyte surface1, as well as other surface antigens such as H–2, HLA and θ antigens present on murine and human lymphocytes2–4. The present study was undertaken to demonstrate the relationship between the receptors of two plant lectins, con A with specificity for glucoside residues, and wheat germ agglutinin (WGA) with specificity for N-acetyl-glucosamine residues5, that are both present on the surface of murine thymocytes.
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LUSTIG, S., PLUZNIK, D. Inhibition and reversal of wheat germ agglutinin-induced caps by concanavalin A. Nature 260, 148–150 (1976). https://doi.org/10.1038/260148a0
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DOI: https://doi.org/10.1038/260148a0
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