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Post-transcription control of isocitrate lyase induction in the eukaryotic alga Chlorella fusca

Nature volume 257, pages 498–501 (1975)Cite this article

A Corrigendum to this article was published on 11 December 1975

Abstract

WE have shown previously1 that poly(A)-containing RNA can be isolated from the green alga Chlorella fusca 8p (ref. 2) and that this RNA has messenger-like properties, as it is polydisperse and stimulates polypeptide synthesis in vitro in a wheat embryo system. We show here that the Chlorella protein isocitrate lyase has been identified as a product of in vitro translation of poly(A)-containing RNA, so confirming messenger function in this fraction. We have used the in vitro translation system as a means of assaying for the presence of isocitrate lyase message sequences in poly(A)-containing RNA isolated from Chlorella cultured under different conditions, and we report here that slow-growing non-induced cells may contain high levels of mRNA for this enzyme although the enzyme is not synthesised.

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Author notes

  1. A. H. SCRAGG

    Present address: Microbiological Research Establishment, Porton Down, Salisbury, Wiltshire

Authors and Affiliations

  1. Division of Biochemistry, National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

    A. H. SCRAGG

  2. Botany Department, The Queen's University of Belfast, Belfast, BT7 1NN, UK

    P. C. L. JOHN

  3. Department of Microbiology, Queen Elizabeth College, Campden Hill Road, London, W8 7AH, UK

    C. F. THURSTON

Authors
  1. A. H. SCRAGG
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  2. P. C. L. JOHN
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  3. C. F. THURSTON
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SCRAGG, A., JOHN, P. & THURSTON, C. Post-transcription control of isocitrate lyase induction in the eukaryotic alga Chlorella fusca. Nature 257, 498–501 (1975). https://doi.org/10.1038/257498a0

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