Abstract
WE wish to propose an explanation for the heterogeneity shown1 by ribosomal precursor RNAs on gel electrophoresis. The clearest demonstration of this phenomenon is shown in Fig. 1. 32P-labelled RNA was eluted from single gel slices taken across the peak, and rerun in the presence of 3H-labelled marker. The 32P-RNA samples run in their original positions and not coincidentally with the marker, that is, the width of the peak does not result from diffusion alone.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Loening, U. E., Grierson, D., Rogers, M. E., and Sartirana, M. L., in Functional Units in Protein Biosynthesis (edit. by Cox, R. A., and Hadjilov, A. A.) 395–405, (FEBS Symposia 23, 1972).
Loening, U. E., Biochem. J., 102, 251–257 (1967).
Loening, U. E., Jones, K., Birnstiel, M. L., J. molec. Biol., 45, 353–366 (1969).
Tiollais, P., Galibert, F., and Boiron, M., Proc. natn. Acad. Sci. U.S.A., 68, 1117–1120 (1971).
Loening, U. E., Biochem. J., 113, 131–138 (1969).
Landesmann, R., and Gross, P. R., Devl Biol., 19, 244–260 (1969).
Retel, J., and Planta, R. J., Eur. J. Biochem, 3, 248–258 (1967).
Retel, J., and Planta, R. J., Biochim. biophys. Acta, 224, 458–469 (1970).
Slack, J. M. W., and Loening, U. E., Eur. J. Biochem., 43, 59–67 (1974).
Staynoff, D. Z., Pinder, J. C., and Gratzer, W. B., Nature new Biol., 235, 108–110 (1972).
Sypherd, P. S., J. molec. Biol., 56, 311–318 (1971).
Fresco, J. R., Alberts, B. M., and Doty, P., Nature, 188, 98–101 (1960).
Spirin, A. S., Prog. Nucl. Acid Res. (Mol. Biol.), 1, 301–346 (1963).
Fresco, J. R., Adams, A., Ascione, R., Henley, D., and Lindhal, T., Cold Spring Harb. Symp. quant. Biol., 31, 527–537 (1966).
Aubert, M., Scott, J. F., Reynier, M., and Monier, R., Proc. natn. Acad. Sci., U.S.A., 61, 292–299 (1968).
Jordan, B. R., J. molec. Biol., 55, 423–439 (1971).
Richards, E. G., Lecanidou, R., and Geroch, M. E., Eur. J. Biochem., 34, 262–267 (1973).
Cramer, F., Prog. Nucl. Acid Res., (Mol Biol.), 11, 391–421 (1971).
Arnott, S., Prog. Biophys. Mol. Biol., 22, 179–213 (1971).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
SLACK, J., SARTIRANA, ML. & LOENING, U. Multiple conformations of ribosomal precursor RNA. Nature 253, 282–284 (1975). https://doi.org/10.1038/253282a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/253282a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
