Abstract
THE ribosomal RNA genes of Escherichia coli are arranged in tandem behind a common promotor in the order of 16S, 23S, 5S (for review see ref. 1). In this report we redetermine the positions of the 16S (0.55 × 106 dalton), 23S (1.10 × 106 dalton), and 5S rRNA (0.04 × 106 dalton) cistrons on their unit of transcription (scripton) by the ultraviolet light mapping technique2–9 principally to demonstrate the applicability of this technique to the determination of the transcriptional linkage of genes in E. coli.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Pace, N. R., Bact. Rev., 37, 562 (1973).
Starlinger, P., and Kölsch, E., Biochem. Biophys. Res. Commun., 17, 508 (1964).
Van den Bos, R. C., Rètel, J., and Planta, R. J., Biochim. biophys. Acta, 232, 494 (1971).
Herrlich, P., Rahmsdorf, H. J., Pai, S. H., and Schweiger, M., Proc. natn. Acad. Sci. U.S.A., 71, 1088 (1974).
Bräutigam, A., and Sauerbier, W., J. Virol., 12, 882 (1973).
Bräutigam, A., and Sauerbier, W., J. Virol., 13, 1110 (1974).
Hackett, P. B., thesis, Univ. Colarado (1974).
Hercules, K., and Sauerbier, W., J. Virol., 12, 872 (1973).
Hercules, K., and Sauerbier, W., J. Virol., 12, 341 (1974).
Michalke, H., and Bremer, H., J. molec. Biol., 41, 1 (1969).
Sauerbier, W., Millette, R., and Hackett, P., Biochim. biophys. Acta, 209, 368 (1970).
Sibatani, A., and Mizuno, N., Biochim. biophys. Acta, 76, 188 (1963).
Kroes, H. H., Schepman, A.-M., and Rörsch, A., Biochim. biophys. Acta, 76, 201 (1963).
Matsuzaki, K., and Nozu, K., Biochim. biophys. Acta, 186, 85 (1969).
Kossman, C. R., thesis, Univ. Colorado (1973).
Dunn, J. J., and Studier, F. W., Proc. natn. Acad. Aci. U.S.A., 70, 3296 (1973).
Nikolaev, N., Silengo, L., and Schlessinger, D., Proc. natn. Acad. Sci. U.S.A., 70, 3361 (1973).
Setlow, J. K., Boling, M. E., and Bollum, F. J., Proc. natn. Acad. Sci. U.S.A., 53, 1430 (1965).
Brdar, B., Drakulic, M., and Kos, E., Biochim. biophys. Acta, 119, 362 (1966).
Bleyman, M., Kondo, M., Hecht, N., and Woese, C., J. Bact., 99, 535 (1969).
Bleyman, M., and Woese, C., Proc. natn. Acad. Sci. U.S.A., 63, 532 (1969).
Webb, K., and Bleyman, M., Nature new Biol., 237, 79 (1972).
Sauerbier, W., Adv. Radiat. Biol. (in the press).
Hill, R. F., Biochim. biophys. Acta., 30, 636 (1958).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
HACKETT, P., SAUERBIER, W. Radiological mapping of the ribosomal RNA transcription unit in E. coli. Nature 251, 639–641 (1974). https://doi.org/10.1038/251639a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/251639a0
This article is cited by
-
Radiation sensitivity of messenger RNA
Molecular and General Genetics MGG (1979)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.