Abstract
THE clonal rat myoblast cell line L6 isolated by Yaffe1 undergoes myogenesis when the mononucleated myoblasts become confluent and fuse into multinucleated myotubes. This morphological differentiation is correlated with the synthesis of characteristic muscle proteins such as myosin and creatine kinase2. When L6 myoblasts fuse the specific activity of adenylate cyclase3 decreases and cyclic AMP, dibutyryl cyclic AMP and theophylline reversibly inhibit the differentiation process4. In primary cultures of rat muscle cells, a decrease in cyclic AMP has been reported at the time of fusion5. These facts support the hypothesis that low levels of cyclic AMP correlate with myoblast differentiation, while high levels inhibit fusion. In view of the relationship between (the action of insulin and the biosynthesis of cyclic nucleotides6 and the effects of the hormone on muscle metabolism7, we have examined the influence of insulin on myogenesis of cultured L6 myoblasts. The hormone replaces partially the serum requirement for morphological differentiation of primary cultures of chick embryo myoblasts8,9,, and now we have found that it stimulates fusion and the specific activity of creatine kinase in L6 myoblasts in the presence or absence of serum.
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References
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MANDEL, JL., PEARSON, M. Insulin stimulates myogenesis in a rat myoblast line. Nature 251, 618–620 (1974). https://doi.org/10.1038/251618a0
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DOI: https://doi.org/10.1038/251618a0
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