Abstract
SENSITISATION of lymphocytes in vitro facilitates the study of cellular immune mechanisms under conditions in which the exact input of reactive cells and antigen is controlled. Such systems have been used to generate cytotoxic effector cells against alloantigens1–3, and more recently against tumour-associated antigens (TAA) in animal models4,5. Less work has been done on TAA induction of immune responses using human antigens and effector cells. Golub et al.6 demonstrated in vitro sensitisation against antigens present on autochthonous Burkitt lymphoma cells. Since such sensitised cells mediate tumour or allograft rejection reactions in vivo7,8, the in vitro ‘education’ of lymphocytes against TAA is an appealing potential modality for immunotherapy of human cancer. Several preliminary clinical trials have been reported in which ‘educated’ lymphoid cells were administered to cancer patients9,10, although the clinical benefits of this procedure remain uncertain. We describe here culture conditions that increase cytotoxic activity of melanoma patients' lymphocytes against melanoma-derived target cell lines.
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GOLUB, S., MORTON, D. Sensitisation of lymphocytes in vitro against human melanoma-associated antigens. Nature 251, 161–163 (1974). https://doi.org/10.1038/251161a0
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DOI: https://doi.org/10.1038/251161a0
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