Abstract
DURING serial undiluted passage of simian virus 40 (SV40) in monkey kidney cells, the viral genome undergoes genetic rearrangements involving the incorporation of cellular DNA sequences1–5 as well as deletions and reiterations of specific viral DNA sequences3,6–8. In previous work7, a mutant of an SV40 variant (DAR; refs 9–11) was identified after serial passage of DAR virus in primary green monkey kidney cells. This mutant DNA was physically mapped by hybridising it to specific DNA segments produced by restriction endonuclease cleavage of SV40 DNA8. The mutant DNA was found to contain only one-third of the SV40 genome which was reiterated three times to produce a DNA molecule the size of wild-type SV40 (3.2×106 daltons). Most of the early gene sequences and a portion of the late gene region12 were deleted from the triplicated segment, but it retained the normal site for initiation of viral DNA replication8.
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DAVOLI, D., FAREED, G. Amplification of a circular segment of SV40 DNA. Nature 251, 153–155 (1974). https://doi.org/10.1038/251153a0
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DOI: https://doi.org/10.1038/251153a0
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