Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Operator and promoter are separate in the tryptophan operon in Salmonella typhimurium

Abstract

Studies of the trp mRNA made by Salmonella typhimurium strains carrying trpOA and trpOAB multisite mutations have shown that these are deletions. They do not extend into the trp promoter (trpP1) as shown by their ability to recombine with trpP4 mutation. Thus the trp operator (trpO) and the trp promoter are seperable and arranged in the order P1-O-A.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Callahan III, R., Blume, A. J., and Balbinder, E., J. molec. Biol., 51, 709, (1970).

    Article  CAS  Google Scholar 

  2. Jordan, E., Saedler, H., and Starlinger, P., Molec. gen. Genet., 102, 353 (1968).

    Article  CAS  Google Scholar 

  3. Shapiro, J. A., J. molec. Biol., 40, 93 (1969).

    Article  CAS  Google Scholar 

  4. Balbinder, E., Blume, A. J., Weber, A., and Tamaki, H., J. Bact., 95, 2217 (1968).

    CAS  PubMed  Google Scholar 

  5. La Scolea, L. J., and Balbinder, E., J. Bact., 112, 877 (1972).

    CAS  PubMed  Google Scholar 

  6. Balbinder, E. Callahan III, R., McCann, P. P., Cordaro, J. C., Weber, A. R., Smith, A. M., and Angelosanto, F., Genetics, 66, 31 (1970).

    CAS  PubMed  PubMed Central  Google Scholar 

  7. Denney, R. M., and Yanofsky, C., J. molec. Biol., 64, 319 (1972).

    Article  CAS  Google Scholar 

  8. Imamoto, F., and Yanofsky, C., J. molec. Biol., 28, 1 (1967).

    Article  CAS  Google Scholar 

  9. Imamoto, F., Morikawa, N., and Sato, K., J. molec. Biol., 13, 169 (1965).

    Article  CAS  Google Scholar 

  10. Ito, J., and Crawford, I. P., Genetics, 52, 1303 (1965).

    CAS  PubMed  PubMed Central  Google Scholar 

  11. Blume, A. J., and Balbinder, E., Genetics, 53, 577 (1966).

    CAS  PubMed  PubMed Central  Google Scholar 

  12. Rose, J. K., Mosteller, R. D., and Yanofsky, C., J. molec. Biol., 51, 541 (1970).

    Article  CAS  Google Scholar 

  13. Hiraga, S., and Yanofsky, C., Nature new Biol., 237, 47 (1972).

    Article  CAS  Google Scholar 

  14. Cordaro, J. C., and Balbinder, E., Genetics, 67, 151 (1971).

    CAS  PubMed  PubMed Central  Google Scholar 

  15. Demerec, M. E., Adelberg, E. A., Clark, A. J., and Hartman, P. E., Genetics, 54, 61 (1966).

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Vogel, H. J., and Bonner, D. M., J. biol. Chem., 218, 97 (1956).

    CAS  PubMed  Google Scholar 

  17. Okamoto, K., Sugino, Y., and Nomura, M., J. molec. Biol., 5, 527 (1962).

    Article  CAS  Google Scholar 

  18. Morse, D. A., and Yanofsky, C., J. molec. Biol., 41, 317 (1969).

    Article  CAS  Google Scholar 

  19. Gillespie, D., and Spiegelman, S., J. molec. Biol., 12, 829 (1965).

    Article  CAS  Google Scholar 

  20. Smith, O. H., and Yanofsky, C., in Methods in enzymology (edit. by Colowick, S. P., and Kaplan, N. O.) 5, 794 (Academic Press, New York, 1962).

    Google Scholar 

  21. Bauerle, R. H., and Margolin, P., Proc. natn. Acad. Sci. U.S.A., 56, 111 (1966).

    Article  ADS  CAS  Google Scholar 

  22. Blume, A. J., Weber, A., and Balbinder, E., J. Bact., 95, 2230 (1968).

    CAS  PubMed  Google Scholar 

  23. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J., J. biol. Chem., 193, 265 (1951).

    CAS  Google Scholar 

  24. Smith, H. O., and Levine, M., Virology, 31, 207 (1967).

    Article  CAS  Google Scholar 

  25. Benzinger, R., and Hartman, P. E., Virology, 18, 614 (1962).

    Article  CAS  Google Scholar 

  26. Grieshaber, M., and Bauerle, R. H., Nature new biol., 236, 232 (1972).

    Article  Google Scholar 

  27. Margolin, P., and Bauerle, R. H., Cold Spring Harbor Symp. quant. Biol., 31, 311 (1966).

    Article  CAS  Google Scholar 

  28. Leive, L., Biochem. biophys. Res. Commun., 18, 13 (1965).

    Article  CAS  Google Scholar 

  29. Mosteller, R. D., and Yanofsky, C., J. molec. Biol., 48, 525 (1970).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aono, H., Angelosanto, F. & Balbinder, E. Operator and promoter are separate in the tryptophan operon in Salmonella typhimurium. Nature 251, 119–123 (1974). https://doi.org/10.1038/251119a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/251119a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing