Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Detection and radioassay of soluble circulating immune complexes using guinea pig peritoneal exudate cells

Nature volume 248, pages 156–159 (1974)Cite this article

Abstract

METHODS at present available for the detection and quantitation of soluble circulating immune complexes include precipitation with C1q in agarose gel diffusion1, precipitation with monoclonal rheumatoid factor9, ultracentrifugation, inhibition of antibody–mediated lymphocyte cytotoxicity10, and precipitation with an optimal concentration of polyethylene glycol2,3. These procedures either involve the use of substances isolated by fairly laborious means, or take some time to yield results. Preliminary tests in this laboratory showed that aggregated human immunoglobulin ingested by guinea pig macrophages was easily detectable by immunofluorescence, and that similar inclusions could be demonstrated after exposure of macrophages to systemic lupus erythematosus (SLE) sera.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Agnello, V., Carr, R. I., Koffler, D., and Kunkel, H. G., Med. Proc., 28, 2447 (1969).

    Google Scholar 

  2. Creighton, W. D., Lambert, P. H., and Miescher, P. A., J. Immun., 4, 1219 (1973).

    Google Scholar 

  3. Harrington, J. C., Fenton, II, J. W., and Pert, J. H., Immunochemistry, 18, 413 (1971).

    Article  Google Scholar 

  4. Hunter, W. M., and Greenwood, F. C., Nature, 194, 495 (1962).

    Article  ADS  CAS  Google Scholar 

  5. Lay, W. H., and Nussenzweig, V., J. Immun., 102, 1172 (1969).

    CAS  PubMed  Google Scholar 

  6. Mancini, G., Carbonara, A. O., and Heremans, J. F., Immunochemistry, 2, 235 (1965).

    Article  CAS  Google Scholar 

  7. Mantovani, B., Rabinovitch, M., and Nussenzweig, V., J. exp. Med., 135, 780 (1972).

    Article  CAS  Google Scholar 

  8. Shortman, K., Williams, N., and Adams, P., J. Immun. Meth., 1, 273 (1972).

    Article  CAS  Google Scholar 

  9. Winchester, R. J., Kunkel, H. G., and Agnello, V., J. exp. Med., 134, 2868 (1971).

    Google Scholar 

  10. Jewell, D. P., and Maclennan, I. C. M., Clin. exp. immun., 14, 219 (1973).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. MRC Rheumatism Research Unit, Canadian Red Cross Memorial Hospital, Taplow, Maidenhead, Berkshire

    I. I. ONYEWOTU, E. J. HOLBOROW & G. D. JOHNSON

Authors
  1. I. I. ONYEWOTU
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E. J. HOLBOROW
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. D. JOHNSON
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

ONYEWOTU, I., HOLBOROW, E. & JOHNSON, G. Detection and radioassay of soluble circulating immune complexes using guinea pig peritoneal exudate cells. Nature 248, 156–159 (1974). https://doi.org/10.1038/248156a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/248156a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing