Abstract
IN previous papers it has been shown that the in vitro template activity of DNA1 and chromatin2 with DNA polymerase I of Escherichia coli is altered by the action of ionising radiation. These changes have been attributed1 to the formation of new binding sites on the DNA for the enzyme, some sites being active in the synthesis of DNA and others inactive. These new sites are associated with single-strand breaks in the DNA. By working under conditions either of enzyme excess or of excess of template it is possible to distinguish between newly formed active and inactive enzyme binding sites.
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SAFFHILL, R., COOPER, H. & ITZHAKI, R. Detection by DNA polymerase I of breaks produced in rat liver chromatin in vivo by alklating agents. Nature 248, 153–156 (1974). https://doi.org/10.1038/248153a0
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DOI: https://doi.org/10.1038/248153a0
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