Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Article
  • Published:

Specialised transformation in Escherichia coli K12

Abstract

A specialised transformation system has been developed in E. coli K12 by using specialised transducing phage DNA as donor DNA. Transformation of a marker on a F′ episome has also been achieved.

This is a preview of subscription content, access via your institution

Access options

Buy this article

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Oishi, M., and Cosloy, S. D., Biochem. biophys. Res. Commun., 49, 1568 (1972).

    Article  CAS  Google Scholar 

  2. Cosloy, S. D., and Oishi, M., Proc. natn. Acad. Sci. U.S.A., 70, 84 (1973).

    Article  ADS  CAS  Google Scholar 

  3. Wackernagel, W., Biochem. biophys. Res. Commun., 51, 306 (1973).

    Article  CAS  Google Scholar 

  4. Cosloy, S. D., and Oishi, M., Molec. gen. Genet., 124, 1 (1973).

    Article  CAS  Google Scholar 

  5. Oishi, M., Proc. natn. Acad. Sci. U.S.A., 64, 1292 (1969).

    Article  ADS  CAS  Google Scholar 

  6. Barbour, S. D., and Clark, A. J., Proc. natn. Acad. Sci. U.S.A., 65, 955 (1970).

    Article  ADS  CAS  Google Scholar 

  7. Wright, M., Buttin, G., and Hurwitz, J., J. biol. Chem., 246, 6543 (1971).

    CAS  PubMed  Google Scholar 

  8. Lieberman, R. P., and Oishi, M., Nature new Biol., 243, 75 (1973).

    Article  CAS  Google Scholar 

  9. Matsushiro, A., Sato, K., and Kida, S., Virology, 23, 299 (1964).

    Article  CAS  Google Scholar 

  10. Deeb, S. S., Okamoto, K., and Hall, B. D., Virology, 31, 289 (1967).

    Article  CAS  Google Scholar 

  11. Nishimune, Y., Virology, 53, 236 (1973).

    Article  CAS  Google Scholar 

  12. Grossman, L., Levine, S. S., and Allison, W. S., J. molec. Biol., 3, 47 (1961).

    Article  CAS  Google Scholar 

  13. Yanofsky, C., and Ito, J., J. molec. Biol., 21, 313 (1966).

    Article  CAS  Google Scholar 

  14. Beckwith, J. R., and Signer, E. R., J. molec. Biol., 19, 254 (1966).

    Article  CAS  Google Scholar 

  15. Oka, A., Ozeki, H., and Inselburg, J., Virology, 46, 556 (1971).

    Article  CAS  Google Scholar 

  16. Yamagishi, H., Yoshizako, F., and Sato, K., Virology, 30, 29 (1966).

    Article  CAS  Google Scholar 

  17. Kushner, S. R., Nagaishi, H., Templin, A., and Clark, A. J., Proc. natn. Acad. Sci. U.S.A., 68, 824 (1971).

    Article  ADS  CAS  Google Scholar 

  18. Kaiser, A. D., and Hogness, D. S., J. molec. Biol., 2, 392 (1961).

    Article  Google Scholar 

  19. Corwin, L. M., Fanning, G. R., Feldman, F., and Margolin, P., J. Bact., 91, 1509 (1966).

    CAS  PubMed  Google Scholar 

  20. Wang, C. C., and Newton, A., J. Bact., 98, 1135 (1969).

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oishi, M., Cosloy, S. Specialised transformation in Escherichia coli K12. Nature 248, 112–116 (1974). https://doi.org/10.1038/248112a0

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1038/248112a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing