Abstract
Amoeba proteus has been used with some success in studies of nuclear and cytoplasmic activities where transfer of the nuclei from one cell to another, or comparative studies of anucleate and nucleate cells, allow separation of the activities1,2. This technique, however, answers many questions only partially, for it has been impossible to observe the behaviour of nuclei in the absence of cytoplasm. We report here an initial investigation to obtain a medium in which nuclei can be isolated for short periods without damage.
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References
Ord, M. J., Exp. Cell Res., 53, 73 (1968).
Lorch, I. J., and Jeon, K. W., Nature, 221, 1073 (1969).
Chapman-Andresen, C., and Dick, D. A. T., CR Lab. Carlsberg, 32, 445 (1962).
Riddle, J., Exp. Cell Res., 26, 158 (1962).
Burnstock, N., and Philpot, J. St L., Exp. Cell Res., 16, 657 (1959).
Jeon, K. W., in Methods in Cell Physiology (edit. by Prescott, D. M.), 4 (Academic Press, New York, 1970).
Honda, S., in Methods in Developmental Biology (edit. by Wilt, F. H., and Wessells, N. K.), 646 (Crowell, New York, 1967).
Mittermayer, C., Braun, R., and Rusch, H. P., Biochim. Biophys. Acta, 114, 536 (1966).
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ORD, M., BELL, L. Viability of Isolated Nuclei. Nature 226, 854–855 (1970). https://doi.org/10.1038/226854a0
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DOI: https://doi.org/10.1038/226854a0
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