Abstract
VISUAL pigments are conjugated proteins containing either retinal or 3-dehydroretinal as the chromophoric prosthetic group. The molar absorptivity (ε) of the pigments at their absorption maximum in the visible spectrum has been determined either by measuring the retinal or the apoprotein content in a given amount of visual pigment. Knowledge of the molar absorptivity of visual pigments is of practical importance because concentrations are most easily determined by spectro-photometric methods. In addition, this parameter has a theoretical interest in that it enables one to calculate the quantum yield (φ) of bleaching.
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HELLER, J. Absorptivity and Quantum Yield of Bleaching in Bovine Visual Pigment500. Nature 225, 636–637 (1970). https://doi.org/10.1038/225636c0
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DOI: https://doi.org/10.1038/225636c0
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