Abstract
ISOLATED synaptic vesicles have been clearly shown to bind transmitters of the catecholamine type1. The binding of acetylcholine (ACh) by isolated vesicles has not been substantiated. The report by Burton2 that preparations of synaptic vesicles took up radioactive ACh has been criticized on methodological grounds by Marchbanks3, who demonstrated that 14C–ACh exchanges with nerve ending ACh but not vesicular ACh. Kuriyama et al.4, on the other hand, showed that 14C–ACh binds to a purified vesicle fraction from whole mouse brain. This binding may be considered non-specific in that several organic and inorganic ions could prevent or reverse it.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Kirshner, N., J. Biol. Chem., 237, 234 (1962).
Burton, R. M., Intern. J. Neuropharmacol., 3, 13 (1964).
Marchbanks, R. M., Biochem. J., 106, 87 (1968).
Kuriyama, K., Roberts, E., and Vos, J., Brain Res., 9, 231 (1968).
Barker, L. A., Amaro, J., and Guth, P. S., Biochem. Pharmacol., 16, 2181 (1967).
Whittaker, V. P., Michaelson, I. A., and Kirkland, J., Biochem. J., 92, 91 (1964).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
GUTH, P. Acetylcholine Binding by Isolated Synaptic Vesicles in vitro. Nature 224, 384–385 (1969). https://doi.org/10.1038/224384a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/224384a0
This article is cited by
-
Fine structure and innervation of the avian adrenal gland
Zeitschrift f�r Zellforschung und Mikroskopische Anatomie (1973)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.