Abstract
AN important method of peptide sequence determination, namely mass spectrometry of N-methylated acyl peptides, has been described recently1,2. We devised the same method during studies of gastrin3, and have found that methylation by methyl iodide and silver oxide in dimethylformamide is not straightforward for residues of methionine, glutamic acid and aspartic acid. These more complex reactions, which involve the peptide backbone, do not prevent the method being used, but understanding of them is a prerequisite for its general application.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Das, B. C., Géro, S. D., and Lederer, E., Biochem. Biophys. Res. Commun., 29, 211 (1967).
Das, B. C., Géro, S. D., and Lederer, E., Nature, 217, 547 (1968).
Kenner, G. W., and Sheppard, R. C., Proc. Roy. Soc., B, 170, 89 (1968).
Witkop, B., Adv. Prot. Chem., 16, 265 (1961).
Wilson, C. L., J. Amer. Chem. Soc., 69, 3002 (1947).
Liberek, B., Tetrahedron Lett., 1103 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
AGARWAL, K., JOHNSTONE, R., KENNER, G. et al. Mass Spectrometry of N-Methylated Peptide Derivatives. Nature 219, 498–499 (1968). https://doi.org/10.1038/219498a0
Received:
Issue Date:
DOI: https://doi.org/10.1038/219498a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
