Abstract
IT has already been shown that soluble material can be obtained from human spleens which specifically inhibits lymphocytotoxic human isoantisera directed against transplantation antigens of the HL-A system1,2. The method used in these experiments was based on one which had proved successful in the preparation of mouse H-2 transplantation antigens3, and involved the autolysis of crude spleen cell membranes. We have continued our studies of human H antigens by investigating whether proteolytic enzymes can release specifically active soluble material from crude human spleen cell membranes. This communication describes the use of papain in liberating soluble material and gives details of specificity tests carried out on the enzyme-liberated products. It was found that unseparated material liberated by enzyme digestion showed considerable non-specific inhibition. Material of high specific activity, however, was obtainable by further purification using ion-exchange chromatography.
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References
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SANDERSON, A., BATCHELOR, J. Transplantation Antigens from Human Spleens. Nature 219, 184–186 (1968). https://doi.org/10.1038/219184a0
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DOI: https://doi.org/10.1038/219184a0
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