Abstract
EVIDENCE which has accumulated over the past decade from mammalian and non-mammalian sources about the enzyme(s) involved in DNA synthesis has indicated the possibility of a multiunit structure for this enzyme. Mammalian DNA polymerase DNA nucleotidyl-transferase (E.G.2.7.7.7) was first isolated from a high speed supernatant fraction of cell homogenates, instead of a nuclear fraction where DNA synthesis takes place1. Later it was shown that cytoplasmic fractions, obtained by non-aqueous techniques2 and also by the use of a homogeniza-tion medium containing Ca++ (ref. 3) to retain morphological integrity of nuclei, contained substantial amounts of this enzyme.
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ERHAN, S. Model for DNA Polymerase. Nature 219, 160–162 (1968). https://doi.org/10.1038/219160a0
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DOI: https://doi.org/10.1038/219160a0
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