Abstract
IN experiments to compare the activity of factor XIII (fibrin stabilizing factor) in serum and plasma, it was necessary to activate the factor XIII in plasma by addition of calcium, but to prevent the plasma from clotting. Heparin was therefore used as an anti-coagulant. Addition of heparin to the test system resulted in the factor XIII deficient substrate clot being rendered insoluble in 5 M urea or 1 per cent monochloracetic acid (MCA) without addition of a source of factor XIII. An investigation of the effects of heparin on the solubility of fibrin in these reagents was therefore undertaken.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Laki, K., and Lorand, L., Science, 108, 280 (1948).
Loewy, A. G., Trans. Conf. Intern. Comm. Blood Clotting Factors, Gleneagles, Scotland, 1963, Thromb. Diath. Haemorrh., Suppl. 13, 109 (1964).
Loewy, A. G., Dahleberg, J. E., Dorwart, jun., W. V., Weber, M. J., and Eisele, J., Biochem. Biophys. Res. Commun., 15, 177 (1964).
Buluk, K., Januszko, T., and Olbromski, J., Nature, 191, 1093 (1961).
Loewy, A. G., Dahlberg, A., Dunathan, K., Krid, R., and Wolfinger, jun., H. L., J. Biol. Chem., 236, 2634 (1961).
Sheppard, E., and Wright, I. S., Arch. Biochem. Biophys., 52, 426 (1954).
Temperley, I. J., Irish. J. Med. Sci., 448, 159 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CUDDIGAN, B. Heparin and the Stabilization of Fibrin. Nature 217, 779–780 (1968). https://doi.org/10.1038/217779b0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/217779b0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.