Abstract
WHILE investigating the effect of various tissue extracts on the division of cells stimulated with phytohaemagglutinin (PHA) we noticed that in control cultures with PHA there seemed to be a significant increase in the number of non-viable cells. We recorded the number of viable cells and the total number of cells in thirty-eight subsequent experiments. The viability of cells grown with PHA is important in view of previous observations on the cytotoxic action of lymphocytes after exposure to PHA1,2, and the use of PHA in patients with aplastic anaemias3,4. The changes also affect the quantitation of cell responses in culture.
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PEGRUM, G., BARRY, C. Cell Viability in Leucocyte Cultures stimulated with Phytohaemagglutinin. Nature 216, 802–803 (1967). https://doi.org/10.1038/216802a0
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DOI: https://doi.org/10.1038/216802a0
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