Abstract
Takemoto and Liebhaber described two variants (r and r+) of encephalomyocarditis (EMC) virus which differ in the size of plaques formed in cultures of L cells1. Plaques of r+ are 1 mm or less in diameter; those of r, 8 mm or greater. The small size of the r+ plaques is thought to be caused by sulphated acid polysaccharides in the agar overlay which bind the virus electrostatically and prevent attachment to neighbouring susceptible cells2. Revertants appear when r+ is grown under liquid medium. Approximately 0.5 per cent to 2 per cent of the plaque-forming particles (PFP) in our pools either resemble r variant or form plaques intermediate in size between r+ and r. Growth of this latter revertant (ri) after plaque purification is not inhibited by sulphated acid polysaccharides. Thus, inhibitor-insensitive plaque-forming particles invariably contaminate r+ pools at low dilutions but are not present at high dilutions.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Takemoto, K. K., and Liebhaber, H., Virology, 14, 456 (1961).
Liebhaber, H., and Takemoto, K. K., Virology, 20, 559 (1963).
Craighead, J. E., Fed. Proc., 24, 159 (1965).
Liebhaber, H., and Takemoto, K. K., Virology, 14, 502 (1961).
Craighead, J. E., Proc. Soc. Exp. Biol. and Med., 119, 408 (1965).
McLaren, A., and Sanders, F. K., J. Hygiene, 57, 106 (1959).
Ryser, H. J. P., and Hancock, R., Science, 150, 501 (1965).
De Vries, A., Salgo, J., Matoth, Y., Nevo, A., and Katchalski, E., Arch. Intern. Pharmacodyn., 104, 1 (1965).
Smull, C. E., and Ludwig, E. H., J. Bacteriol., 84, 1035 (1962).
Amos, H., and Kearns, K. E., Exp. Cell Res., 32, 14 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
CRAIGHEAD, J. Polycation Effect on Virulence of Nonpathogenic r+ Variant of the Encephalomyocarditis Virus. Nature 214, 716–717 (1967). https://doi.org/10.1038/214716a0
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1038/214716a0
This article is cited by
-
DEAE-dextran enhancement of in vivo infection with murine leukemia virus
Archiv f�r die gesamte Virusforschung (1973)
-
Effect of histone on oncogenic properties of Moloney's murine sarcoma virus
Archiv f�r die gesamte Virusforschung (1973)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.