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Pulse Labelled RNA associated with Ribosomes in Escherichia coli

Abstract

Pulse-labelling studies involving sucrose gradient centrifugation in changing concentrations of magnesium ions have been used to demonstrate that newly synthesized RNA, contrary to the current view, is not reversibly bound to 70S ribosomes but is attached even in low concentrations of magnesium ions to both 5OS and 30S ribosomal sub-units

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References

  1. Jacob, F., and Monod, J., J. Mol. Biol., 3, 318 (1961).

    Article  CAS  PubMed  Google Scholar 

  2. Gros, F., Hiatt, H., Gilbert, W., Kurland, C. G., Risebrough, R. W., and Watson, J. D., Nature, 190, 581 (1961).

    Article  ADS  CAS  PubMed  Google Scholar 

  3. Monier, R., Naono, S., Hayes, D., Hayes, F., and Gros, F., J. Mol. Biol., 5, 311 (1962).

    Article  CAS  PubMed  Google Scholar 

  4. Artman, M., Fry, M., and Engelberg, H. (in preparation).

  5. Artman, M., and Engelberg, H., Biochim. Biophys. Acta, 80, 517 (1964).

    CAS  PubMed  Google Scholar 

  6. Artman, M., and Engelberg, H., Biochim. Biophys. Acta, 95, 687 (1965).

    Article  CAS  PubMed  Google Scholar 

  7. Davis, B. D., and Mingioli, E. S., J. Bacteriol., 60, 17 (1950).

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Weller, D. L., and Horowitz, J., Biochim. Biophys. Acta, 87, 361 (1964).

    CAS  PubMed  Google Scholar 

  9. Morgan, R. S., J. Mol. Biol., 4, 115 (1962).

    Article  CAS  PubMed  Google Scholar 

  10. McCarthy, B. J., Britten, R. J., and Roberts, R. B., Biophys. J., 2, 57 (1962).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Midgley, J. E. M., Biochim. Biophys. Acta, 61, 513 (1962).

    CAS  Google Scholar 

  12. Gilbert, W., J. Mol. Biol., 6, 374 (1963).

    Article  CAS  PubMed  Google Scholar 

  13. Wettstein, F. O., Staehelin, T., and Noll, H., Nature, 197, 430 (1963).

    Article  ADS  CAS  PubMed  Google Scholar 

  14. Kiho, Y., and Rich, A., Proc. U.S. Nat. Acad. Sci., 51, 111 (1964).

    Article  ADS  CAS  Google Scholar 

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ARTMAN, M., SILMAN, N. & ENGELBERG, H. Pulse Labelled RNA associated with Ribosomes in Escherichia coli. Nature 213, 39–42 (1967). https://doi.org/10.1038/213039a0

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