Relationship between Human Luteinizing Hormone and Human Chorionic Gonadotropin

Abstract

PREVIOUS analysis of a rabbit hyperimmune serum to sheep luteinizing hormone (LH) showed seven components which cross-reacted with sheep LH and follicle stimulating hormone (FSH), bovine thyroid stimulating hormone (TSH), as well as pregnant mare's serum (PMS) and human chorionic gonadotropin (HCG)¹. The hormone was injected subcutaneously in Freund's complete adjuvant in weekly doses of 1 mg for 6 weeks, and, after a 10 mg booster dose in the seventh week, the animal was rested and bled for the first time in the tenth week. Thereafter, with insignificant variations, the animal was given 1 mg of hormone every other week and bled in the weekly interval between injections. Certain samples from between the twenty-eighth and fifty-second bleedings were pooled and used in the experiments. At the beginning of this period a total dose of 51 mg LH had been given over a period of 14 months. This communication is concerned especially with the cross-reaction of this antiserum with HCG and HLH and the relationship which this shows between the two hormone preparations.