Abstract
THE involvement of phospholipids in the adenosine triphosphatase activity of human erythrocytes was demonstrated by Schatzmann1, who found that treatment with phospholipase C reduced the activity of both the Mg2+ and the Na+–plus–K+–stimulated ATPase. Tatibana2 and Ohniishi3 reported a specific inactivation of the Na+–plus–K+ stimulated ATPase after treatment of ghosts with phospholipase A. The lipids present in freeze-dried red cell ghosts can be distinguished as so-called loosely and strongly bound fractions4,5. The loosely bound fraction, extracted by ether treatment, contains all the sterols and about 23 per cent of the phospholipids from the human erythrocytes, while the strongly bound lipids, obtained after treatment with ethanol–ether (3 : 1 v/v) consist of phospholipids only5. We have investigated the involvement of lipid fractions in the ATPase activities.
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ROELOFSEN, B., BAADENHUYSEN, H. & VAN DEENEN, L. Effects of Organic Solvents on the Adenosine Triphosphatase Activity of Erythrocyte Ghosts. Nature 212, 1379–1380 (1966). https://doi.org/10.1038/2121379a0
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DOI: https://doi.org/10.1038/2121379a0
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