Abstract
FOLLOWING the observation that the DNA and RNA of synchronized cells of Chlorella which have been given pulses of phosphorus-32 in inorganic phosphate are rapidly labelled in certain fractions1, we have performed analogous experiments on normal and anucleate cells of Acetabularia. This organism provides a very convenient system for investigation of the synthesis of RNA outside the nucleus, perhaps in the plastids. When we applied the technique of pulse-labelling to cells of Acetabularia crenulata we found that most of the label was incorporated in polyphosphate. We also investigated the distribution of total nucleic acids in normal and anucleate cells and the occurrence of pulse-labelled RNA fractions.
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Richter, G., and Senger, H., Biochim. Biophys. Acta, 95, 362 (1965).
Hämmerling, J., Arch. Protistenkunde, 97, 7 (1944).
Beth, K., Z. Naturforsch., 8 b, 334 (1953).
Richter, G., and Senger, H., Biochim. Biophys. Acta, 87, 502 (1964).
Janowski, M., Biochim. Biophys. Acta, 103, 399 (1965).
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RICHTER, G. Pulse-labelling of Nucleic Acids and Polyphosphates in Normal and Anucleate Cells of Acetabularia. Nature 212, 1363 (1966). https://doi.org/10.1038/2121363a0
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DOI: https://doi.org/10.1038/2121363a0
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