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One Dimensional Resolution of Nucleic Acid Bases on Cellulose Layers

Nature volume 212, pages 1359–1360 (1966)Cite this article

Abstract

RESOLUTION of nucleic acid derivatives on cellulose layers1–5 has advantages over paper chromatography, particularly in the speed of separation of mixtures. Keck and Hagen5 have separated the five common bases of nucleic acids one dimensionally on cellulose layers, using as solvent a mixture of methanol, water and hydrochloric acid, but the resolution of uracil and thymine is inferior to that achieved in paper chromatography6. Randerath7 achieves a more sensitive separation, but uses two solvents. The present communication describes a rapid one dimensional procedure which has been used in this department for the separation of the five common bases found in perchloric acid hydrolysates6 of RNA and DNA mixtures.

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References

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Authors and Affiliations

  1. Department of Chemistry and Applied Chemistry, Royal College of Advanced Technology, Salford

    A. B. HARRIS & R. WARBURTON

Authors
  1. A. B. HARRIS
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  2. R. WARBURTON
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HARRIS, A., WARBURTON, R. One Dimensional Resolution of Nucleic Acid Bases on Cellulose Layers. Nature 212, 1359–1360 (1966). https://doi.org/10.1038/2121359b0

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  • DOI: https://doi.org/10.1038/2121359b0

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