Abstract
THE chemical methods available for preparing cytidine diphosphate ethanolamine (CDP-ethanolamine) either give a poor yield of the product1 or require rather elaborate procedures2,3. In the method described here, use has been made of the enzyme CTP : ethanolamine-phosphate cytidylyltransferase (EC. 2.7.7.14) from rat liver, to prepare cytidine-5′-diphosphate-(2-14C)-ethanolamine and cytidine-5′-(β-32P)-diphosphate ethanolamine in high yield. This enzyme, which was originally described by Kennedy and Weiss4, is present exclusively in the particle-free supernatant from a tissue homogenate5,6.
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References
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Letters, R., and Michelson, A. M., Bull. Soc. Chim. Biol., 45, 1353 (1963).
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CHOJNACKI, T., METCALFE, R. Enzymatic Synthesis of Labelled Cytidine-5′-Diphosphate Ethanolamine. Nature 210, 947–948 (1966). https://doi.org/10.1038/210947a0
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DOI: https://doi.org/10.1038/210947a0
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