Abstract
A PROBLEM of great interest to the clinician is the diagnosis of thalassaemia trait. An objective and specific test for detection of this disease was first offered by Kunkel et al.1,2 who, using starch block electrophoresis, found that in cases of thalassaemia trait the A2 haemoglobin was characteristically elevated. This fraction has been extensively investigated by electrophoresis using paper3, starch block4,5, starch gel6, cellulose acetate7, and acrylamide gel8 as the supporting media. Both starch block and starch gel electrophoresis are widely used to-day for haemoglobin analysis but are difficult to standardize and require a great deal of ‘laboratory technique’. Acrylamide gel is superior to starch in that it is easier to work with, is more reproducible, is faster arid the fractions can be quantitated by direct densitometry9,10.
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FERRIS, T., EASTERLING, R. & BUDD, R. Quantitative Determination of Haemoglobin A2 by Acrylamide-gel Electrophoresis. Nature 208, 1103–1104 (1965). https://doi.org/10.1038/2081103a0
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DOI: https://doi.org/10.1038/2081103a0
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