Abstract
Rapp and Borsos1, in their study of the effects of low ionic strength on immune haemolysis, using sheep cells and guinea-pig complement, found that the erythrocytes were haemolysed in the absence of antibody at extremely low ionic strength. The authors gave details for the preparation of a number of isotonic sucrose buffers of various ionic strengths, and the buffers used in this work were those described by these authors.
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References
Rapp, H. J., and Borsos, T., J. Immunol., 91, 826 (1963).
Stratton, F., Gunson, H. H., and Rawlinson, V. I., Transfusion, 2, 135 (1962).
Muller-Eberhard, H. J., and Kunkel, H. G., Proc. Soc. Exp. Biol. and Med., 106, 291 (1961).
Hinz, C. F., and Mollner, A. M., J. Immunol., 91, 512 (1963).
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STRATTON, F., RAWLINSON, V. Interaction between Human Serum Complement and Normal Human Red Cells at Low Ionic Strength. Nature 207, 305–306 (1965). https://doi.org/10.1038/207305a0
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DOI: https://doi.org/10.1038/207305a0
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