Abstract
UNTIL now only alkaline fast-green staining1 has been used to any extent for the histochemical detection of basic nuclear proteins. For the determination of DNA, the Feulgen reaction as well as gallocyanin chromalum (GC) staining have proved successful2. The amino and guanido groups of proteins react stoichiometrically with metaphosphoric acid3,4. After pretreating cells with metaphosphoric acid it is possible to bind a basic dyestuff like GC indirectly to basic groups of proteins. By comparing biochemical and histochemical data, it can be shown that fast-green staining at pH. 8.2 as well as the GC-metaphosphate technique give closely similar results for histones.
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JOBST, K., SANDRITTER, W. Cytophotometric Determinations of Basic Proteins of Cell Nuclei with Basic Dyes. Nature 206, 204 (1965). https://doi.org/10.1038/206204a0
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DOI: https://doi.org/10.1038/206204a0
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