Abstract
IN this communication we report the presence of glycolyl-neuraminic acid in sialic acids from ox brain gangliosides. Our method of analysis consisted of the following six steps: (i) gangliosides were extracted from ox brain grey matter and purified according to a method which we have previously described1; (ii) gangliosides so obtained were hydrolysed in 0.1 N sulphuric acid at 80° C for 2 h, to split off sialic acid; (iii) sialic acid was purified on ‘Dowex’ 2 × 8 column as acetate according to Svennerholm2, and desalted on ‘Amberlite IR–120’; (iv) the solution was dried in vacuum and the residue was hydrolysed in 1 N sulphuric acid for 5 h at 100° C to obtain glycolic acid3; (v) glycolic acid was purified on ‘Dowex’ 2 × 8 column as acetate and eluted with NaCl 2 M; (vi) the glycolic acid was identified by the Eegriwe reaction4 and by gas-chromatography.
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References
Tettamanti, G., Bertona, L., Berra, B., and Zambotti, C., Giornale Ital. Biochem., 13, 326 (1964).
Svennerholm, L., Biochim. Biophys. Acta, 14, 604 (1957).
Tettamanti, G., et al., Istituto Lombardo (rend. Sc.), B, 97, 157 (1963).
Eegriwe, E., Z. Anal. Chem., 89, 123 (1932).
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TETTAMANTI, G., BERTONA, L., BERRA, B. et al. Glycolyl-neuraminic Acid in Ox Brain Gangliosides. Nature 206, 192 (1965). https://doi.org/10.1038/206192a0
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DOI: https://doi.org/10.1038/206192a0
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