Abstract
IN work recorded here, rats were injected intraperitoneally with 8 ml. of a 50 per cent sucrose solution. They were killed 2 h after the injection. Some of the properties of the hepatic lysosomes were found to be deeply affected by the treatment, namely: (1) their density was reduced, as shown by a shift towards the regions of low density in the distribution curves of their hydrolases, following density equilibration in a glycogen gradient (Fig. 1); (2) their size was increased, as suggested by a fall of the sedimentation boundaries of their enzymes in the centrifuge tube, after incomplete sedimentation in a stabilizing gradient of aqueous sucrose (Fig. 2). On the other hand, the distribution of proteins, used as marker for mitochondria1 and of particle-bound catalase, remained practically unaltered.
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References
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WATTIAUX, R., WATTIAUX-DE CONINCK, S., RUTGEERTS, MJ. et al. Influence of the Injection of a Sucrose Solution on the Properties of Rat-liver Lysosomes. Nature 203, 757–758 (1964). https://doi.org/10.1038/203757a0
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DOI: https://doi.org/10.1038/203757a0
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