Abstract
ENZYMES in cell-free extracts of Thiobacillus thioparus catalyse the reduction of thiosulphate to sulphide and sulphite. Sulphite is then oxidized to sulphate and adenosine diphosphate produced in a coupled substrate phosphorylation which is not inhibited by 2:4-dinitrophenol1,2. Comparison of the dinitrophenol inhibition of carbon dioxide fixation when either thiosulphate or sulphide was oxidized by intact cells of T. thioparus suggested that both dinitrophenol-sensitive and dinitrophenol-insensitive phosphorylations were coupled to thiosulphate oxidation3. We now present direct evidence from ATP analyses which shows that phosphorylation coupled to thiosulphate oxidation is much less sensitive to dinitro-phenol inhibition than that coupled to sulphide oxidation.
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References
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KELLY, D., SYRETT, P. Inhibition of Formation of Adenosine Triphosphate in Thiobacillus thioparus by 2 : 4-Dinitrophenol. Nature 202, 597–598 (1964). https://doi.org/10.1038/202597a0
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DOI: https://doi.org/10.1038/202597a0
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