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A Role for Peroxidase in Biosynthesis of Auxin

Abstract

THE metabolic formation of indolyl-3-acetic acid from tryptophan has been well established1,2. The intermediate compounds in this conversion have not been identified. A recent review2 has indicated that several pathways can be formulated that are consistent with the results obtained by use of tryptophan labelled with carbon-14. Recently we have demonstrated the oxidative formation of 3-methylthiopropionamide from methionine in the presence of purified horse-radish peroxidase, Mn++ and pyridoxal-5′-phosphate3. We have now extended this observation to tryptophan and have found that, under oxidative conditions with the foregoing cofactors, peroxidase will convert tryptophan to indole-3-acetamide.

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References

  1. Van Overbeek, J., Bot. Rev., 25, 267 (1959).

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  2. Wightman, F., Canad. J. Bot., 40, 689 (1962).

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  3. Mazelis, M., and Ingraham, L. L., J. Biol. Chem., 237, 109 (1962).

    CAS  PubMed  Google Scholar 

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RIDDLE, V., MAZELIS, M. A Role for Peroxidase in Biosynthesis of Auxin. Nature 202, 391–392 (1964). https://doi.org/10.1038/202391a0

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