Abstract
Jacobs and Harris1 have shown that at temperatures above 37° the viability of cultures of Escherichia coli and Staphylococcus aureus which were damaged by phenol declined markedly, and subsequent investigations2 showed that the optimum temperature for the growth of 3 strains of phenol-treated E. coli was in the region of 30°, It seemed probable that the techniques of counting using pour plates or roll tubes, in which the inoculum is exposed to molten agar at 45°, would be unsatisfactory for such damaged cells, and it was decided to test this hypothesis.
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References
Jacobs, S. E., and Harris, N. D., J. Appl. Bact., 23, 294 (1960).
Harris, N. D., and Whitefield, M. (to be published).
Harris, N. D., Richards, J. P., and Whitefield, M., J. Appl. Bact., 24, 182 (1961).
Miles, A. A., and Misra, S. S., J. Hyg. (Camb.), 38, 732 (1938).
Jacobs, S. E., and Harris, N. D., Congr. Intern. Microbiol, Rome, 1, 603 (1953).
Harris, N. D., and Richards, J. P., Nature, 192, 87 (1961).
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HARRIS, N., WHITEFIELD, M. A Lethal Effect on Damaged Eschierichia coli associated with the Counting Technique. Nature 200, 606–607 (1963). https://doi.org/10.1038/200606a0
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DOI: https://doi.org/10.1038/200606a0
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