Electrophoretic Behaviour of Rabbit Serum Amylase

Abstract

NOT only is serum amylase heterogeneous in origin, but electrophoretic patterns of enzyme activity appear to be also species specific. The majority of amylase activity in normal human serum, reportedly exhibits electrophoretic mobilities similar to either albumin and γ-globulin^1,2 or γ-globulin alone³. Normal canine serum electrophoretic patterns show greater amounts of amylase activity in the γ-globulin position as compared with the albumin zone². Electrophoresis of normal mouse serum discloses that amylase is largely confined to bands occupied by β₁- and β₂-globulins⁴. Normal rat serum yields a somewhat different pattern in that amylase activity is located principally in the β-globulin area with much less amounts of enzyme in the γ-globulin zone⁵. In order to elucidate the amylase distribution in the serum of still another experimental animal., electrophoretic patterns were established for normal rabbits.