Abstract
IN a previous paper1 we described a method for the detection of small amounts of ketosteroids on chromatograms by means of DNPH. The subsequent removal of the background colour enables the use of this method for quantitative analysis, as previously suggested. A satisfactory solvent system, by which the hydrazones were eluted from strips and then determined by spectro-photometry, was found. The procedure described here requires little time, and is suitable for routine estimations. We have used this method in metabolic investigations in vitro as well as for measurement of the progesterone concentration in sheep's ovarian venous blood. In both cases very satisfactory results were obtained.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Stupnicki, R., and Stupnicka, E., J. Chromatog., 9, 235 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
STUPNICKI, R., STUPNICKA, E. Quantitative Analysis of Ketosteroids by Means of Paper Chromatography. Nature 200, 165–166 (1963). https://doi.org/10.1038/200165a0
Issue Date:
DOI: https://doi.org/10.1038/200165a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
