Abstract
IN work directed toward the separation of several volatile 2-halogenated carboxylic acids by paper chromatography, it was found desirable to develop these acids, not, as is usually the case, in the form of their salts1, but as the free acids. The so-called ‘swamp acid’ technique2 used for this purpose simply involves the use of a relatively large quantity of some acid as part of the solvent system, usually acetic or formic. This acid is necessary to repress ionization of the unknown acid, which would otherwise give rise to ‘tails’ or ‘ghosts’. However, it soon became clear in working with the 2-halogenated acids that removal of the ‘swamp acid’ preparatory to spraying with the more common acid-base indicators also removed the volatile unknown acids. Therefore, some scheme had to be found for locating the unknowns in the presence of the ‘swamp acid’.
Similar content being viewed by others
Article PDF
References
Kennedy, E. P., and Barker, H. A., Anal. Chem., 23, 1033 (1951). Reid, R. L., and Lederer, M., Biochem. J., 50, 60 (1951).
Lugg, J. W. H., and Overell, B. T., Australian J. Sci. Res., A, 1, 98 (1948).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MEIKLE, R. Paper Chromatography of 2-Halogenated Carboxylic Acids : N,N-Dimethyl-p-phenylazoaniline as an Acid-Base Indicator Reagent. Nature 196, 61 (1962). https://doi.org/10.1038/196061a0
Issue Date:
DOI: https://doi.org/10.1038/196061a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.