Abstract
THE reaction of double-stranded deoxyribonucleic acids (DNA) and mercuric compounds1 proceeds by two principal steps2–4. In the DNA's occurring naturally, these steps can be demonstrated and defined by the presence of isobestic points in the ultraviolet spectra of the equilibrium mixture. The first reaction occurs in the region 0 ⩽ r ⩽ 0.5 – 0.6; the second, in the region r ⩾ 0.5 – 0.6, where, as usual, the quantity r is defined as the number of moles of HgII added per mole of nucleotide.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Katz, S., J. Amer. Chem. Soc., 74, 2238 (1952).
Thomas, C. A., J. Amer. Chem. Soc., 76, 6032 (1954).
Pour-El, A., thesis, Univ. Calif., Berkeley (1960) in the laboratory of C. A. Dekker.
Yamane, T., and Davidson, N., J. Amer. Chem. Soc., 83, 2599 (1961).
Katz, S., Nature, 194, 569 (1962).
Katz, S., Biochim. Biophys. Acta (to be published)
Ferreira, R., Ben-Zvi, E., Yamane, T., Vasilevskis, J., and Davidson, N., in Advances in the Chemistry of the Co-ordination Compounds, 457 (Macmillan Co., New York, 1961).
Cf., for example, Klotz, I., in The Proteins, edit. by Neurath, H., and Bailey, K., 1, Pt. B, 790 (Academic Press, New York, 1953).
Dove, W. F., and Yamane, T., Biochem. Biophys. Res. Comm., 3, 608 (1960).
Katz, S., and Santilli, V., Biochim. Biophys. Acta, 55, 621 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
KATZ, S. Reversible Reaction of Double-Stranded Polynucleotides and HgII : Separation of the Strands. Nature 195, 997–998 (1962). https://doi.org/10.1038/195997a0
Issue Date:
DOI: https://doi.org/10.1038/195997a0
This article is cited by
-
Der physikalische Status der Deoxyribonucleinsäure im Bakteriennucleoid
Antonie van Leeuwenhoek (1965)
-
Zum chemischen Status der Deoxyribonucleins�ure im Bakteriennucleoid
Archiv f�r Mikrobiologie (1965)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
