Abstract
A VARIETY of methods, including use of immunological or isotopic markers or the excretion-rate of products of hæmoglobin catabolism, have been used to estimate in vivo viability of red blood cells1. In measuring the viability of cells subjected to storage or other in vitro treatment, it is necessary to calculate survival using an independent measurement of dilution since the experimental cells may in part be rapidly lost2,3. The dilution volume, or red cell mass, of the recipient has been estimated indirectly from plasma volume and hæmatocrit determinations3 or directly by use of simultaneous or sequential injections of control red cells marked with a labelling system different from that used for the experimental cells2,4.
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Strumia, M. M., Progress in Hematology, 1, 74 (1956). Root, W. S., Methods in Medical Research, 8, 1 (1960).
Gibson, J. G., Aub, J. C., Evans, R. D., Peacock, W. C., Levine, jun., J. W., and Sack, T., J. Clin. Invest., 26, 704 (1947).
Strumia, M. M., Taylor, L., Sample, A. B., Colwell, L. C., and Dugan, A., Blood, 10, 429 (1955).
Mollison, P. L., and Veall, N., Brit. J. Hæmat., 1, 62 (1955). Gibson, J. G., and Scheitlin, W. A., J. Lab. and Clin. Med., 46, 679 (1955). Donohue, D. M., and Finch, C. A., Vox Sang., 2, 369 (1957).
Sterling, K., and Grey, S. J., J. Clin. Invest., 29, 1604 (1950). Read, R. C., New England J. Med., 250, 1021 (1954). Reilly, W. A., French, R. M., Lau, F. Y. K., Scott, K. G., and White, W. E., Circulation, 9, 571 (1954). Baker, C. H., and Remington, J. W., Amer. J. Physiol., 198, 906 (1960).
Jones, N. C. H., Mollison, P. L., and Robinson, M. A., Proc. Roy. Soc., 147 B, 476 (1957).
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DOEBBLER, G., DWYER, R. & RINFRET, A. A Simple Comparative Method of measuring Red Cell Viability. Nature 195, 912–913 (1962). https://doi.org/10.1038/195912a0
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DOI: https://doi.org/10.1038/195912a0
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