Abstract
IT has been shown by Cox1 that pregnane-3α,17α,-20α-triol (pregnanetriol) and related compounds react with 70 per cent ortho-phosphoric acid to produce a brilliant fluorescence. Herman and Bruton2,3 have shown that pregnanetriol and related compounds and digitalis compounds react intensely with antimony pentachloride, which thus can be used as a paper chromatographic detecting agent. Since pregnanetriol reacts with both phosphoric acid and antimony pentachloride, by analogy, those steroids which react strongly with antimony pentachloride could be expected to react with 70 per cent ortho-phosphoric acid. Heftmann et al.4 have noted briefly that aniline-phosphoric acid is useful as a detecting agent for cardiac glycosides.
Similar content being viewed by others
Article PDF
References
Cox, R. I., J. Biol. Chem., 234, 1693 (1959).
Herman, R. H., and Bruton, J., Nature, 190, 444 (1961).
Herman, R. H., Nature, 190, 268 (1961).
Heftmann, E., Berner, P., Hayden, A. L., Miller, H. K., and Mosettig, E., Arch. Biochem. Biophys., 51, 329 (1954).
Herman, R. H., Clayton, L. E., and Bruton, J., Nature, 193, 169 (1962).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
BRUTON, J., HERMAN, R. & CLAYTON, L. Effect of Phosphoric Acid on Digitalis Compounds. Nature 195, 904 (1962). https://doi.org/10.1038/195904a0
Issue Date:
DOI: https://doi.org/10.1038/195904a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.