Abstract
RECENTLY it was shown that the polarographic method is suitable for the determination of small amounts (0.2 per cent and more) of non-virus protein in tobacco mosaic virus (TMV) preparations1. In further experiments we have found this method equally suitable for the detection of traces of protein contaminants in the infective ribonucleic acid (RNA) preparations, isolated from TMV by the phenol method according to Gierer and Schramm2.
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References
Ruttkay-Nedecký, G., Biochem. Biophys. Acta, 26, 455 (1957). Coll. Czechoslov. Chem. Comm. (in the press).
Gierer, A., and Schramm, G., Z. Naturforsch., 11, b, 138 (1956).
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Franklin, R. E., Klug, A., and Holmes, K. C., Ciba Found. Symp. Nature of Viruses (Churchill, Ltd., London, 1957).
Anderer, F. A., Z. Naturforsch., 14, b, 24, 642 (1959).
Gierer, A., Z. Naturforsch., 13, b, 477, 485 (1958).
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RUTTKAY-NEDECKÝ, G., ŠPÁNIK, V. A Sensitive Polarographic Test for the Detection of Protein Impurities in Viral Nucleic Acid. Nature 195, 735–736 (1962). https://doi.org/10.1038/195735a0
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DOI: https://doi.org/10.1038/195735a0
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