Abstract
RECENTLY, Perretta and Thomson1 published a report on the marked stimulatory action exerted by a preparation of erythropoietin on the incorporation of formate labelled with carbon-14 into the bases of spleen and liver-slice nucleic acids. This same preparation, they reported, had no such action on marrow suspensions incubated under the same conditions. Since this finding appeared to offer a useful approach to the biochemical examination of the mechanism of action of erythropoietin and might also furnish a rapid sensitive in vitro assay for the hormone we undertook a confirmatory investigation. Instead of the extract of alcohol-precipitated urine from anæmic rabbits we used a partially purified sheep plasma fraction, with the results summarized here. These results do not confirm the findings of Perretta and Thomson.
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Perretta, M. A., and Thomson, R. Y., Nature, 190, 912 (1961).
White, W. F., Gurney, C. W., Goldwasser, E., and Jacobson, L. O., Rec. Prog. Hormone Res., 16, 219 (Academic Press, Inc., New York, 1960).
Smellie, R. M. S., Thomson, R. Y., and Davidson, J. N., Biochim., Biophys. Acta, 29, 59 (1958).
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DUKES, P., GOLDWASSER, E. Lack of Effect of Plasma Erythropoietin on Formate Incorporation into nucleic Acids in vitro. Nature 195, 1222 (1962). https://doi.org/10.1038/1951222a0
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DOI: https://doi.org/10.1038/1951222a0
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