Abstract
THE development of the kinetic studies of virus biosynthesis requires an accurate and rapid method for determining the amount of virus nucleoprotein contained in small samples of tissue. Bancroft and Curtis1 described a simple method which utilized trichloroacetic acid (TCA) as the substance for precipitating and extracting virus nucleic acid. Although this method is rather rapid, it results in the destruction of virus nucleoprotein and demands careful attention to the final concentration of TCA, because TCA itself has a high absorbance at 260 mµ which may cause gross errors in the measurement of the small amount of viruses. The disadvantages of the method were overcome by using ammonium sulphate instead of TCA: the accuracy and rapidity of the method were also increased.
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References
Bancroft, J. B., and Curtis, R. W., Phytopath., 47, 79 (1957).
Reddi, K. K., Biochim. Biophys. Acta, 24, 238 (1957).
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TANIGUCHI, T. A Rapid Method for Microanalytical Determination of the Amount of Tobacco Mosaic Virus in Plant Tissues. Nature 194, 708 (1962). https://doi.org/10.1038/194708a0
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DOI: https://doi.org/10.1038/194708a0
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