Abstract
1-CM. squares of full-thickness skin were aseptically excised from adult male guinea pigs. 10 days later explants of granulation tissue laid down in the wounds were cultured on plasma clot by the flying coverslip method. 36–48 hr. later, cultures were fixed intact in buffered osmium tetroxide or potassium permanganate, dehydrated with ethanol, and embedded in ‘Araldite’. Parts of the fibroblast outgrowth were sectioned and examined in a Siemen's ‘Elmiskop I’ electron microscope.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Robertson, J. D., Biochem. Soc. Symp., 16, 3 (1959).
Gray, E. G., in Electron Microscopy in Anatomy (Edward Arnold, London, 1961).
Karrer, H. K., J. Biophys. Biochem. Cytol., 7, 181 (1960).
Vogel, A., Fourth Intern. Conf. Electron Microscopy, Berlin, 2, 286 (1958).
Gray, E. G., and Whittaker, V. P., Brit. Med. Bull. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
DEVIS, R., JAMES, D. Electron Microscopic Appearance of Close Relationships between Adult Guinea Pig Fibroblasts in Tissue Culture. Nature 194, 695–696 (1962). https://doi.org/10.1038/194695b0
Issue Date:
DOI: https://doi.org/10.1038/194695b0
This article is cited by
-
Contact inhibition in tissue culture
In Vitro (1970)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
