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Embedding Large Slices of Plant Tissue for Electron Microscopy

Abstract

THE most suitable impregnating resins for ultra-thin sectioning of plant tissue penetrate very slowly to the centre of large masses of cells. This has restricted the range of problems which can be investigated. The following simple technique, an adaptation of that suggested by Luft1, was evolved to embed and section successfully pieces of plant tissue, both meristematic and vacuolated, up to 1 mm.3.

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References

  1. Luft, J. H., J. Biophys. Biochem. Cytol., 9, 409 (1961).

    Article  CAS  Google Scholar 

  2. Millonig, G., J. Biophys. Biochem. Cytol., 11, 736 (1961).

    Article  CAS  Google Scholar 

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JUNIPER, B. Embedding Large Slices of Plant Tissue for Electron Microscopy. Nature 194, 1296–1297 (1962). https://doi.org/10.1038/1941296b0

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