Quantisation of Antibody Hæmagglutinin Activity using Electronic Cell Counting

Abstract

AGGLUTINATION is the most characteristic and widely used reaction for detecting antigen–antibody reactions of red blood cells. The reaction itself is highly sensitive and specific. The quantitation of agglutinin activity generally has been limited to the use of two-fold serial dilution methods, and since such methods are adequate for most routine clinical determinations there has been little interest in the development of quantitative methods. Where more detailed information is desired, the serial dilution methods are inadequate and a procedure of sensitivity and precision would facilitate the examination of a variety of problems related to human blood groups which at present cannot be attempted.