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Preparation of Substrates for Assay of Sulphotransferases

Nature volume 193, pages 1180–1181 (1962)Cite this article

Abstract

INVESTIGATIONS have shown1–3 that enzymatic synthesis of ‘active sulphate’ (PAPS*) requires two enzymes. The first enzyme ATP-sulphurylase catalyses the displacement of pyrophosphate from ATP with formation of APS. The second reaction catalysed by APS-kinase is the phosphorylation of the 3′-hydroxyl group of APS by ATP to form PAPS.

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Authors and Affiliations

  1. Agricultural Chemistry Section, Chemistry Department, The University, Glasgow, W.2

    LIAM M. FOGARTY & W. RONALD REES

Authors
  1. LIAM M. FOGARTY
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  2. W. RONALD REES
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FOGARTY, L., REES, W. Preparation of Substrates for Assay of Sulphotransferases. Nature 193, 1180–1181 (1962). https://doi.org/10.1038/1931180a0

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