Abstract
THE erythropoietic activity in the plasma of rabbits made anæmic with phenylhydrazine can be isolated as an acid glycoprotein. A dialysed and lyophilized ethanol fraction from heat-coagulated plasma consistently gives us a potent stable highly water-soluble preparation. Six laboratories have found good correlation in a comparative assay of a batch prepared by us1. Further di-ethyl-amino-ethyl cellulose column fractionation removes 40–50 per cent of inert protein and results in the active glycoprotein previously described2.
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Keighley, G., Lowy, P. H., Borsook, H., Goldwasser, E., Gordon, A. S., Prentice, T. C., Rambach, W. A., Stohlman, jun., F., and Van Dyke, D. C., Blood, 16, 1424 (1960).
Lowy, P. H., Keighley, G., and Borsook, H., Nature, 185, 102 (1960).
Morgan, W. T. J., and Partridge, S. M., Biochem. J., 35, 1140 (1941).
Westphal, O., Lüderitz, O., and Bister, F., Zeitschr. f. Naturforsch., 7, B, 148 (1952).
Kirby, K. S., Biochem. J., 64, 405 (1956).
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LOWY, P., KEIGHLEY, G. Use of Phenol in the Isolation of Erythropoietic Glycoprotein. Nature 192, 75 (1961). https://doi.org/10.1038/192075a0
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DOI: https://doi.org/10.1038/192075a0
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